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Lior Pachter
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Lior S. Pachter
Person information
- affiliation:University of California, Berkeley, USA
2020 – today
- 2024
[j53]
Delaney K. Sullivan, Lior Pachter:
Flexible parsing, interpretation, and editing of technical sequences with splitcode.Bioinform.40(6) (2024)[j52]
Tara Chari, Gennady Gorin, Lior Pachter:
Biophysically interpretable inference of cell types from multimodal sequencing data.Nat. Comput. Sci.4(9): 677-689 (2024)- 2023
[j51]
Ángel Gálvez-Merchán, Kyung Hoi (Joseph) Min, Lior Pachter, A. Sina Booeshaghi:
Metadata retrieval from sequence databases with ffq.Bioinform.39(1) (2023)[j50]
Laura Luebbert, Lior Pachter:
Efficient querying of genomic reference databases with gget.Bioinform.39(1) (2023)[j49]
Tara Chari, Lior Pachter:
The specious art of single-cell genomics.PLoS Comput. Biol.19(8) (2023)- 2022
[j48]
Gennady Gorin, Meichen F•
Abstract
Simple Summary
Next-generation sequencing (NGS) is a powerful tool used in genomics research. NGS can sequence millions of DNA fragments at once, providing detailed information about the structure of genomes, genetic variations, gene activity, and changes in gene behavior. Recent advancements have focused on faster and more accurate sequencing, reduced costs, and improved data analysis. These advancements hold great promise for unlocking new insights into genomics and improving our understanding of diseases and personalized healthcare. This review article provides an overview of NGS technology and its impact on various areas of research, such as clinical genomics, cancer, infectious diseases, and the study of the microbiome.
Abstract
The advent of next-generation sequencing (NGS) has brought about a paradigm shift in genomics research, offering unparalleled capabilities for analyzing DNA and RNA molecules in a high-throughput and cost-effective manner. This transformati
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Revisão Revisado por paresInfluenza genome analysis using pyrosequencing method: current applications for a moving target
2009;Taylor & Francis;Volume: 9;Issue: 5Linguagem: Inglês
10.1586/erm.09.21
ISSN1744-8352
AutoresVarough Deyde, Larisa V. Gubareva,
Tópico(s)Biological Agents for Bioterrorism
ResumoPyrosequencing is a high-throughput non-gel-based DNA sequencing method that was introduced in the late 1990s. It employs a DNA sequencing-by-synthesis approach based on real-time measurement of pyrophosphate released from incorporation of dNTPs. A cascade of enzymatic reactions proportionally converts the pyrophosphate to a light signal recorded in a form of peaks, known as pyrograms. Routinely, a 45–60-nucleotide sequence is obtained per reaction. Recent improvements introduced in the assay chemistry have extended the read to approximately 100 nucleotides. Since its advent, pyrosequencing has been applied in the fields of microbiology, molecular bi